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Modulation of Golgi‐associated microtubule nucleation throughout the cell cycle
Author(s) -
Maia Ana Rita Ramada,
Zhu Xiaodong,
Miller Paul,
Gu Guoqiang,
Maiato Helder,
Kaverina Irina
Publication year - 2013
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.21079
Subject(s) - interphase , golgi apparatus , mitosis , telophase , microbiology and biotechnology , biology , cell plate , cell cycle , microtubule nucleation , microtubule organizing center , microtubule , cell , cell division , cytokinesis , anaphase , centrosome , biochemistry , endoplasmic reticulum
A microtubule (MT) subpopulation that emanates from Golgi membrane has been recently shown to comprise a significant part of MT network in interphase cells. In this study, we address whether Golgi membrane, which is being extensively remodeled throughout the cell cycle, retains its ability to nucleate MTs at diverse cell cycle stages. Live cell imaging and immunofluorescence microscopy reveals that Golgi‐derived MTs form at multiple stages of the cell cycle, including G 1 , G 2 , and distinct phases of mitosis. However, the capacity of Golgi to nucleate MTs in mitosis is strongly down‐regulated as compared with interphase, indicating that this property is cell cycle regulated. We demonstrate that Golgi‐derived MTs are indispensable for efficient Golgi assembly in telophase, and speculate that these noncentrosomal MTs may hold specific functions at other cell cycle stages. © 2012 Wiley Periodicals, Inc