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A role for central spindle proteins in cilia structure and function
Author(s) -
Smith Katherine R.,
Kieserman Esther K.,
Wang Peggy I.,
Basten Sander G.,
Giles Rachel H.,
Marcotte Edward M.,
Wallingford John B.
Publication year - 2011
Publication title -
cytoskeleton
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.95
H-Index - 86
eISSN - 1949-3592
pISSN - 1949-3584
DOI - 10.1002/cm.20498
Subject(s) - midbody , ciliogenesis , biology , cilium , cytokinesis , microbiology and biotechnology , intraflagellar transport , basal body , microtubule , function (biology) , cell division , septin , genetics , cell , mutant , flagellum , gene
Cytokinesis and ciliogenesis are fundamental cellular processes that require strict coordination of microtubule organization and directed membrane trafficking. These processes have been intensely studied, but there has been little indication that regulatory machinery might be extensively shared between them. Here, we show that several central spindle/midbody proteins (PRC1, MKLP‐1, INCENP, centriolin) also localize in specific patterns at the basal body complex in vertebrate ciliated epithelial cells. Moreover, bioinformatic comparisons of midbody and cilia proteomes reveal a highly significant degree of overlap. Finally, we used temperature‐sensitive alleles of PRC1 / spd‐1 and MKLP‐1 / zen‐4 in C. elegans to assess ciliary functions while bypassing these proteins' early role in cell division. These mutants displayed defects in both cilia function and cilia morphology. Together, these data suggest the conserved reuse of a surprisingly large number of proteins in the cytokinetic apparatus and in cilia. © 2010 Wiley‐Liss, Inc.