Open AccessInducing precocious anaphase in cultured mammalian cells
Author(s) -
Canman Julie C.,
Salmon E.D.,
Fang Guowei
Publication year - 2002
Publication title -
cell motility and the cytoskeleton
Language(s) - English
Resource type - Journals
eISSN - 1097-0169
pISSN - 0886-1544
DOI - 10.1002/cm.10032
Subject(s) - anaphase , prometaphase , biology , spindle checkpoint , microbiology and biotechnology , securin , cytokinesis , anaphase promoting complex , cell cycle checkpoint , kinetochore , separase , aurora b kinase , cell cycle , genetics , cell division , chromosome , cell , gene
The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate this checkpoint, preventing anaphase and subsequent cell cycle events. This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells. Cell Motil. Cytoskeleton 52:61–65, 2002. © 2002 Wiley‐Liss, Inc.