z-logo
open-access-imgOpen Access
Heterogeneity of magnitude, allergen immunodominance, and cytokine polarization of cockroach allergen‐specific T cell responses in allergic sensitized children
Author(s) -
Silva Antunes Ricardo,
Sutherland Aaron,
Frazier April,
Schulten Veronique,
Pomés Anna,
Glesner Jill,
Calatroni Agustin,
Altman Matthew C.,
Wood Robert A.,
O'Connor George T.,
Pongracic Jacqueline A.,
Khurana Hershey Gurjit K.,
Kercsmar Carolyn M.,
Gruchalla Rebecca S.,
Gill Michelle,
Liu Andrew H.,
Zoratti Edward,
Kattan Meyer,
Busse Paula J.,
Bacharier Leonard B.,
Teach Stephen J.,
Wheatley Lisa M.,
Togias Alkis,
Busse William W.,
Jackson Daniel J.,
Sette Alessandro
Publication year - 2021
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1002/clt2.12073
Subject(s) - immunology , medicine , aeroallergen , immunoglobulin e , allergy , t cell , asthma , peripheral blood mononuclear cell , immunodominance , cytokine , cytotoxic t cell , interleukin 4 , allergen , immune system , antibody , biology , in vitro , biochemistry
Background Characterization of allergic responses to cockroach (CR), a common aeroallergen associated with asthma, has focused mainly on IgE reactivity, but little is known about T cell responses, particularly in children. We conducted a functional evaluation of CR allergen‐specific T cell reactivity in a cohort of CR allergic children with asthma. Methods Peripheral blood mononuclear cells (PBMCs) were obtained from 71 children, with mild‐to‐moderate asthma who were enrolled in a CR immunotherapy (IT) clinical trial, prior to treatment initiation. PBMC were stimulated with peptide pools derived from 11 CR allergens, and CD4+ T cell responses assessed by intracellular cytokine staining. Results Highly heterogeneous responses in T cell reactivity were observed among participants, both in terms of the magnitude of cytokine response and allergen immunodominance. Reactivity against Bla g 9 and Bla g 5 was most frequent. The phenotype of the T cell response was dominated by IL‐4 production and a Th2 polarized profile in 54.9% of participants, but IFNγ production and Th1 polarization was observed in 25.3% of the participants. The numbers of regulatory CD4+ T cells were also highly variable and the magnitude of effector responses and Th2 polarization were positively correlated with serum IgE levels specific to a clinical CR extract. Conclusions Our results demonstrate that in children with mild‐to‐moderate asthma, CR‐specific T cell responses display a wide range of magnitude, allergen dominance, and polarization. These results will enable examination of whether any of the variables measured are affected by IT and/or are predictive of clinical outcomes.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here