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Use of Olive Mill Wastewater as a Growth Medium for Superoxide Dismutase and Catalase Production
Author(s) -
Değirmenbaşı Didem,
Takaç Serpil
Publication year - 2018
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201700228
Subject(s) - catalase , superoxide dismutase , chemistry , food science , wastewater , bioremediation , antioxidant , sugar , laccase , enzyme , pulp and paper industry , biochemistry , biology , environmental engineering , bacteria , environmental science , engineering , genetics
Microbial processing of food industrial waste, olive mill wastewater (OMW), is a promising route from not only a bioremediation point of view but also for bio‐refinery concept. Enzymes are among numerous products to be valorised from OMW. In this study, production of microbial antioxidant enzymes in OMW is successfully demonstrated for the first time. Superoxide dismutase (SOD) and catalase (CAT) activities by Rhodotorula glutinis cultivated in OMW in parallel with the biodegradation of wastewater are investigated. The effects of different carbon and nitrogen supplements as well as metal ions added to cultivation medium, temperature, and stress agents are examined to increase SOD and CAT activities. The results show that R. glutinis can grow and produce SOD and CAT in OMW medium. Ethanol as a carbon supplement and urea as a nitrogen supplement provide high enzyme activities. Low temperature stimulates SOD and CAT activities. Mg 2+ is the most effective metal ion for both enzyme activities. Treatment of cells with H 2 O 2 increases SOD and CAT activities whereas UV light did not affect the activities considerably. At all conditions, more than 75% decrease in total phenol concentration and about 75% consumption of reducing sugar presented in cultivation medium are obtained.