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Optimization of Polyhydroxybutyrate Production by Bacillus safensis EBT1
Author(s) -
Madhumathi Ramasamy,
Muthukumar Karuppan,
Velan Manickam
Publication year - 2016
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201500163
Subject(s) - polyhydroxybutyrate , bagasse , yeast extract , chemistry , biomass (ecology) , fourier transform infrared spectroscopy , gel permeation chromatography , food science , substrate (aquarium) , fermentation , bacteria , biology , microbiology and biotechnology , organic chemistry , chemical engineering , polymer , agronomy , ecology , genetics , engineering
The present study deals with the enhanced production of polyhydroxybutyrate (PHB) using a novel potent strain. On the basis of morphological, biochemical, and 16S rRNA analysis, the strain was identified as Bacillus safensis EBT1. The presence of PHB in the cells was identified and affirmed using fluorescent microscopy and Fourier transform infrared spectroscopy. The molecular weight of PHB was ascertained by gel permeation chromatography. Under optimized conditions, B. safensis EBT1 showed maximum PHB production and growth with glucose as the carbon source, yeast extract as the nitrogen source, and sugarcane bagasse as the substrate. The process parameters were optimized using central composite design: pH 7, 45 °C and 48 h incubation time. At these conditions, highest biomass concentration of 7.54 g/L and PHB production of 6.41 g/L were observed.