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Interaction between Microbes DNA and Atrazine in Black Soil Analyzed by Spectroscopy
Author(s) -
Zhang Ying,
Wang Zhigang,
Guo Huosheng,
Meng Dongfang,
Wang Yang,
Wong Pokeung
Publication year - 2015
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201400006
Subject(s) - hyperchromicity , ethidium bromide , atrazine , chemistry , fluorescence , intercalation (chemistry) , dna , quenching (fluorescence) , nucleic acid , genotoxicity , biophysics , photochemistry , biochemistry , inorganic chemistry , biology , organic chemistry , physics , toxicity , quantum mechanics , pesticide , agronomy
It could be shown that atrazine bound to microbes DNA in black soil by using ethidium bromide (EB) as a fluorescence probe. To study the interaction between atrazine and the microbes DNA in black soil, spectral absorption and fluorescence analysis were adopted. Atrazine, when bound to DNA, showed a hyperchromic and red shift in the absorption spectra and fluorescence quenching (>50%) in the fluorescence spectra, which indicated the intercalation. The effect of phosphate ion showed that there were electrostatic attractions with the anionic sugar‐phosphate backbone of DNA. The ratio plots “fluorescence intensities in the absence” to “fluorescence intensities in the presence of a quencher” ( F o / F ) showed that the quenching of fluorescence by atrazine was a combined quenching process. The results indicated that there were two binding modes, intercalation and electrostatic attractions, happened simultaneously. The hyperchromicity in the absorption spectra indicated the breaking or denaturation of DNA, and a fluorescence inhibiting effect showed that atrazine had a high ability to bind to the DNA of black soil microbes. Based on these results, it was speculated that the binding between atrazine and the DNA the black soil microbes could inhibit some functions of the nucleic acid, restrain microbial activity or cause genotoxicity.