Premium
Controllable Switching of Enzyme Activity by Poly( N ‐isopropylacrylamide)‐Based Microgels Through Mineralization of Calcium Carbonate in High Pressure CO 2
Author(s) -
Yang Xiaoxi,
Zhang Xiaogang,
Shang Wenting,
Zhang Shujuan
Publication year - 2016
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201300967
Subject(s) - lipase , chemical engineering , self healing hydrogels , chemistry , mineralization (soil science) , composite number , polymer , hydrolysis , candida antarctica , catalysis , materials science , polymer chemistry , enzyme , composite material , nitrogen , organic chemistry , engineering
Stimuli‐responsive hydrogels with high strength and toughness have received great interest in recent years. In this study, temperature‐responsive composite hydrogels involved the Candida antarctica lipase B were prepared by using sodium alginate and poly( N ‐isopropylacrylamide), and were evaluated as catalysts using the model reaction of hydrolysis of p ‐nitrophenol butyrate. The experimental results demonstrated that enzymatic catalyst immobilized in the thermoresponsive carriers offered possibilities for external switching and manipulation for objective reaction system. The mechanical properties of the enzyme–polymer composites pretreated by high pressure carbon dioxide could be significantly improved. The number of recycling use was significantly increased. This work provided a new approach to smart regulation for enzymatic reaction.