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Experimental Investigation and Kinetic Modeling of p ‐Nitrophenol and Phenol by Kissiris‐Immobilized Ralstonia eutropha in a Batch Reactor
Author(s) -
Motamedi Mahdi,
Habibi Alireza,
Maleki Mohammad,
Vahabzadeh Farzaneh
Publication year - 2015
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201300635
Subject(s) - ralstonia , phenol , bioreactor , aeration , degradation (telecommunications) , substrate (aquarium) , chemistry , kinetic energy , nitrophenol , batch reactor , kinetics , nuclear chemistry , chromatography , organic chemistry , biology , physics , catalysis , enzyme , telecommunications , ecology , computer science , quantum mechanics
Co‐metabolic degradation of p ‐nitrophenol (PNP) and phenol by kissiris‐immobilized Ralstonia eutropha cells was studied in a batch bioreactor operated in recycling mode (30°C, initial pH of 7.7, aeration rate of 0.5 vvm, and recycling flow rate of 25 mL min −1 ). Decreasing trends of specific degradation rates of phenol ( q Sg ) and PNP ( q Sng ) with an increase of initial PNP concentration, were mathematically modeled considering the competitive inhibition between growth and non‐growth substrates and self‐inhibition of non‐growth substrate. A non‐linear regression on the equations used in defining of q Sg , and q Sng , was used to estimate the relevant kinetic parameters. On the best fitted curve, the kinetic parameters were q max Sg = 0.032 g Phenol g Cell −1 h −1 , K m Sg = 442.9 mg L −1 , q max Sng = 0.0056 g Phenol g Cell −1 h −1 , K m Sng = 181.2 mg L −1 , and K Sng = 0.489 mg L −1 . These parameters were used for dynamic prediction of the substrates concentration feature during co‐metabolic degradation experiments.