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Screening for and Identification of an Anti‐clam Vibrio Marine Bacterium from an Aquaculture Pond in the Yellow Sea
Author(s) -
Sun Xing,
Zhao Gengmao,
Gu Chenhao,
Liu Ling,
Zhu Ming,
Liu Zhaopu
Publication year - 2016
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201200478
Subject(s) - vibrio alginolyticus , pseudoalteromonas , biology , microbiology and biotechnology , vibrio , aquaculture , probiotic , marine bacteriophage , bacteria , vibrio infections , vibrionaceae , 16s ribosomal rna , vibrio vulnificus , fishery , fish <actinopterygii> , genetics
The identification and use of probiotic bacterial stains is a practical approach to protect clams grown in aquaculture farms from disease. The inhibition of the pathogenic bacterium Vibrio alginolyticus was used as a trait to select a candidate probiotic bacterial strain in this study. An ideal bacterial strain, SW‐1, was isolated from seawater from a clam farm. The selected isolate SW‐1 was identified based on its physiological, morphological, and biochemical characteristics and its 16S rDNA sequence. The experiments showed that strain SW‐1 had a high similarity to Pseudoalteromonas piscicida and could inhibit the growth of V. alginolyticus ( V .‐MP‐1). SW‐1 also improved the survival of clams following challenge with the pathogenic V .‐MP‐1. The mortality of clams was 100% after infection with 10 8 CFU/mL of V. alginolyticus , whereas mortality was only 11% when clams were infected with 10 8 CFU/mL of V .‐MP1 while simultaneously exposed to the same concentration of Pseudoalteromonas SW‐1, indicating that Pseudoalteromonas SW‐1 could be used as a probiotic to protect farmed clams, and thus reduce the effects of antibiotics on aquatic environment.