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Efficiency of Pleurotus florida Laccase on Decolorization and Detoxification of the Reactive Dye Remazol Brilliant Blue R (RBBR) under Optimized Conditions
Author(s) -
Sathishkumar Palanivel,
Balan Kannan,
Palvannan Thayumanavan,
KamalaKannan Seralathan,
Oh ByungTaek,
RodríguezCouto Susana
Publication year - 2013
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201100521
Subject(s) - laccase , chemistry , nuclear chemistry , pleurotus , coomassie brilliant blue , brilliant green , central composite design , response surface methodology , chromatography , enzyme , food science , organic chemistry , staining , medicine , mushroom , pathology
Laccase from the white‐rot fungus Pleurotus florida , produced under solid‐state fermentation conditions, was used for the decolorization of reactive dye Remazol Brilliant Blue R (RBBR). RBBR was decolorized up to 46% by P. florida laccase alone in 10 min. In the presence of N ‐hydroxybenzotriazole (HBT), the rate of decolorization was enhanced 1.56‐fold. Central composite design of response surface methodology with four variables namely, dye, enzyme, redox mediator concentrations, and time at five levels was applied to optimize the RBBR decolorization. The predicted optimum level of variables for maximum RBBR decolorization (87%) was found to be 52.90 mg L −1 (RBBR), 1.87 U mL −1 (laccase), 0.85 mM (HBT), and 7.17 min (time), respectively. The validation results showed that the experimental value of RBBR decolorization (82%) was close to the predicted one. The disappearance of C–N and C–X groups, and a small shift in N–H groups in Fourier‐transform infra red (FTIR) spectroscopy confirms the degradation of RBBR chromophore by laccase enzyme. The phytotoxicity of RBBR was considerably reduced after the treatment with laccase. RBBR decolorization kinetics; K m and V max were calculated to be 145.82 mg L −1 and 24.86 mg L −1 min, respectively.