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Optimization of Minimal Salt Medium for Efficient Phenanthrene Biodegradation by Mycoplana sp. MVMB2 Isolated from Petroleum Contaminated Soil Using Factorial Design Experiments
Author(s) -
Brinda Lakshmi Mahalingam,
Muthukumar Karuppan,
Velan Manickam
Publication year - 2013
Publication title -
clean – soil, air, water
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.444
H-Index - 66
eISSN - 1863-0669
pISSN - 1863-0650
DOI - 10.1002/clen.201000208
Subject(s) - phenanthrene , chemistry , sulfate , magnesium , factorial experiment , potassium , central composite design , nuclear chemistry , phosphate , potassium phosphate , ferrous , salt (chemistry) , ammonium sulfate , inorganic chemistry , response surface methodology , environmental chemistry , chromatography , organic chemistry , statistics , mathematics
Abstract This study presents the degradation of phenanthrene by Mycoplana sp. MVMB2 isolated from petroleum contaminated soil and the media optimization by factorial design experiments. The Plackett–Burman design was used to evaluate the effects of eight variables (potassium dihydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, glucose, inoculum concentration, and phenanthrene concentration) on phenanthrene degradation. Based on the results, the critical medium components having significant influence on the degradation were found to be disodium hydrogen phosphate, magnesium sulfate, ferrous sulfate, and phenanthrene. Furthermore, these four variables were used as central composite design parameters. The optimum minimal salt medium composition obtained by conventional and factorial design experiments for the degradation of phenanthrene by Mycoplana sp. MVMB2 at pH 6.5 and 30°C were found to be, potassium 2.5 g/L dihydrogen phosphate, 0.3505 g/L disodium hydrogen phosphate, 0.5501 g/L magnesium sulfate, 0.02 g/L calcium chloride, 0.0261 g/L ferrous sulfate, 0.6756 g/L phenanthrene, 0.5 g/L glucose, 0.5 g/L ammonium sulfate, and inoculum 5% v/v. The phenanthrene degradation was confirmed by analyzing the metabolites formed.