Premium
Characterization of a Membrane‐Bound O ‐Acetyltransferase Involved in Trioxacarcin Biosynthesis Offers Insights into Its Catalytic Mechanism †
Author(s) -
Yin Yue,
Shen Yi,
Meng Song,
Zhang Mei,
Pan HaiXue,
Tang GongLi
Publication year - 2020
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.202000284
Subject(s) - chemistry , biosynthesis , acyltransferase , acetylation , biochemistry , acetyltransferase , mutagenesis , enzyme , transmembrane protein , acyltransferases , amino acid , active site , stereochemistry , mutant , gene , receptor
Summary of main observation and conclusion Trioxacarcin A (TXN‐A) is a polycyclic aromatic natural product with remarkable biological activity. TxnB11, a membrane‐bound O ‐acetyltransferase involved in TXN‐A biosynthesis that is difficult for protein manipulation, is biochemically characterized here for its acetylation function on C4‐sugar. A proposed catalytic mechanism is supported by transmembrane helix analysis and site‐directed mutagenesis, in which four conserved amino acid residues His35, Ser71, His100 and His317 are essential for enzyme activity. We tested the substrate tolerance of TxnB11 to acyl donors in vitro and found that TxnB11 can also utilize propionyl‐CoA and glycolyl‐CoA, resulting in two new TXN analogs with anti‐tumor activity. This work provides a first understanding of the catalytic mechanism of this unusual acyltransferase family and presents good candidates for creating structural diversity for natural products.