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Radical SAM‐Dependent Adenosylation Involved in Bacteriohopanepolyol Biosynthesis †
Author(s) -
Zhong Yuting,
Ji Xinjian,
Zhang Qi
Publication year - 2020
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201900399
Subject(s) - chemistry , biosynthesis , enzyme , stereochemistry , biochemistry , natural product , superfamily , function (biology) , gene , microbiology and biotechnology , biology
Summary of main observation and conclusion Bacteriohopanepolyols are a group of triterpenoids that play important roles in regulating bacterial cell membrane function. As an intermediate in bacteriohopanepolyol biosynthesis, adenosylhopane production is related to a putative Fe‐S protein HpnH, but the exact role of this enzyme remains unsolved. Here we report characterization of HpnH as a novel radical S‐adenosylmethionine (SAM) superfamily enzyme. In contrast to almost all the members in the superfamily, HpnH does not initiate the reaction by a hydrogen atom abstraction process. Instead, it catalyzes the adenosylation of hopene via a radical addition reaction to produce adenosylhopane, representing the second example of radical SAM‐dependent adenosylation involved in natural product biosynthesis.