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Chemical Protein Synthesis by Native Chemical Ligation and Variations Thereof
Author(s) -
Wang Siyao,
Thopate Yogesh Abaso,
Zhou Qingqing,
Wang Ping
Publication year - 2019
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201900246
Subject(s) - native chemical ligation , chemistry , chemical ligation , ligation , chemical synthesis , combinatorial chemistry , biochemical engineering , nanotechnology , biochemistry , amino acid , microbiology and biotechnology , in vitro , materials science , engineering , biology
For a long time, the total synthesis of proteins was considered as a “mission impossible” because of the tedious and complex synthetic steps and demanding purification processes. However, with the development of modern synthetic methodologies, many protein syntheses have now been reported. More importantly, through chemical synthesis, desired modifications can be installed to target proteins precisely, which is a major advantage over traditional bio‐synthesis approaches. This review summarizes the techniques developed for protein assembly, including native chemical ligation, Se ‐mediated ligation, and a range of other ligation methods. A few synthetic examples, whereby synthetic proteins with desired modifications have been utilized for related biological research, are also included. We believe that chemical synthesis can provide alternative pathways to solve problems that have hitherto proved insurmountable by traditional biological approaches.