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Enzyme‐Triggered Fluorescence Turn‐on: A Probe for Specifically Imaging Ovarian‐Cancer‐Related γ ‐Glutamyltranspeptidase
Author(s) -
Tian Jie,
Yan Qinglong,
Zhu Ying,
Zhang Jichao,
Li Jiao,
Shi Ben,
Xu Ge,
Fan Chunhai,
Zhao Chunchang
Publication year - 2017
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201700248
Subject(s) - chemistry , bodipy , fluorescence , glutathione , chromophore , enzyme , cleavage (geology) , cysteine , ovarian cancer , biochemistry , photochemistry , biophysics , cancer , medicine , physics , geotechnical engineering , quantum mechanics , fracture (geology) , engineering , biology
A fluorescent turn‐on probe for specifically targeting γ ‐glutamyltranspeptidase ( GGT ) was designed and synthesized by integrating boron‐dipyrromethene ( BODIPY ) as a chromophore and glutathione ( GSH ) as the GGT substrate. GGT ‐catalyzed the cleavage of the γ ‐glutamyl bond and generated the aromatic hydrocarbon transfer between the sulfur and the nitrogen atom in BODIPY , leading to distinct optical changes. Such specific responsiveness provides an easily distinguishable fluorescence signal to visualize the GGT activity in living cells and differentiate GGT ‐positive cancer cells from GGT ‐negative cells.