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Amperometric Biosensor for Detection of Phenolic Compounds Based on Tyrosinase, N ‐Acetyl‐ L ‐cysteine‐capped Gold Nanoparticles and Chitosan Nanocomposite
Author(s) -
Dong Wenjuan,
Han Jiyan,
Shi Jia,
Liang Wenting,
Zhang Yuexia,
Dong Chuan
Publication year - 2017
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201600728
Subject(s) - chemistry , biosensor , amperometry , tyrosinase , saturated calomel electrode , catechol , detection limit , nuclear chemistry , colloidal gold , chitosan , nanocomposite , nanoparticle , carbon paste electrode , cysteine , electrode , chromatography , working electrode , electrochemistry , organic chemistry , cyclic voltammetry , nanotechnology , enzyme , biochemistry , materials science
A novel biosensor was fabricated based on the immobilization of tyrosinase and N ‐acetyl‐ L ‐cysteine‐capped gold nanoparticles onto the surface of the glassy carbon electrode via the film forming by chitosan. The NAC‐AuNPs ( N ‐acetyl‐ L ‐cysteine‐capped gold nanoparticles) with the average size of 3.4 nm had much higher specific surface area and good biocompatibility, which were favorable for increasing the immobilization amount of enzyme, retaining the catalytic activity of enzyme and facilitating the fast electron transfer. The prepared biosensor exhibited suitable amperometric responses at −0.2 V for phenolic compounds vs. saturated calomel electrode. The parameters of influencing on the working electrode such as pH , temperature, working potential were investigated. Under optimum conditions, the biosensor was applied to detect catechol with a linear range of 1.0 × 10 −7 to 6.0 × 10 −5 mol•L −1 , and the detection limit of 5.0 × 10 −8 mol•L −1 ( S / N =3). The stability and selectivity of the proposed biosensor were also evaluated.