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Fibrinolytic Evaluation of Compounds Isolated from a Marine Fungus Stachybotrys longispora FG216
Author(s) -
Guo Ruihua,
Zhang Yiting,
Duan Dong,
Fu Qiang,
Zhang Xiangyu,
Yu Xiaowei,
Wang Shujun,
Bao Bin,
Wu Wenhui
Publication year - 2016
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201600623
Subject(s) - chemistry , ergosterol , bioassay , stereochemistry , heteronuclear single quantum coherence spectroscopy , fungus , ethyl acetate , in vitro , two dimensional nuclear magnetic resonance spectroscopy , organic chemistry , biochemistry , botany , genetics , biology
As a part of our continuing work to discover bioactive leading molecules from marine microorganism, ethyl acetate fraction of organic extract of the train Stachybotrys longispora FG216 showed fibrinolytic activity in our primary screen. The bioassay‐guided purification of the active fractions resulted in isolation of a new isoindolone, FGFC2 ( 1 ) (FGFC2, Fungi fibrinolytic compound 2), together with two known compounds, LL‐Z1272β ( 2 ) and ergosterol ( 3 ). The structure of compound 1 was elucidated by the spectral analysis of 1D ( 1 H, 13 C) NMR, 2D (COSY, HSQC, and HMBC) and ESI‐MS. Three compounds were evaluated for fibrinolytic activities in vitro . Compared to FGFC1 (EC 50 47 µmol/L) as a reference drug, compound 1 and ergosterol ( 3 ) showed moderate fibrinolytic activities in vitro with EC 50 values of 108.16 and 156.30 µmol/L, respectively. LL‐Z1272β ( 2 ) had no fibrinolytic activity.