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Specific Electrochemiluminescence of Aptamer‐Functionalized Quantum Dots with Lysozyme and Hemin as Co‐Triggers
Author(s) -
Ji Xubo,
Yao Chuanguang,
Wan Ying,
Song Hongxin,
Xin Peng,
Cui Hongda,
Zheng Chenyu,
Deng Shengyuan
Publication year - 2016
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201500886
Subject(s) - chemistry , lysozyme , aptamer , hemin , electrochemiluminescence , combinatorial chemistry , bioanalysis , detection limit , g quadruplex , substrate (aquarium) , chemiluminescence , nanoparticle , quantum dot , dna , photochemistry , nanotechnology , chromatography , biochemistry , enzyme , heme , genetics , oceanography , materials science , biology , geology
This work proposed a label‐free, cost‐effective and fairly sensitive electrogenerated chemiluminescence (ECL) strategy for the specific detection of lysozyme based on the hemin/G‐quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin‐film as substrate to adsorb thiolated captures: a hairpin‐structured DNA, integrating dual head‐tail connected functional domains: one aptamer sequence for lysozyme and the other for hemin (iron(III) proto ‐porphyrin IX). In the presence of the both, the hairpin conformation unfolded and transformed into the hemin/G‐quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consumption of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 pg·mL −1 to 5 µg·mL −1 , with a detection limit as low as 4.95 pg·mL −1 ( e.g. 9.4 pmol·L −1 ), demonstrating the prospective utilization of DNA technologies in bioanalysis.