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Identification and Fibrinolytic Evaluation of an Isoindolone Derivative Isolated from a Rare Marine Fungus Stachybotrys longispora FG216
Author(s) -
Wang Ge,
Wu Wenhui,
Zhu Quangang,
Fu Shiqing,
Wang Xiaoyu,
Hong Shaotong,
Guo Ruihua,
Bao Bin
Publication year - 2015
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201500176
Subject(s) - chemistry , in vivo , stereochemistry , plasmin , in vitro , fibrinogen , derivative (finance) , isothiocyanate , biochemistry , microbiology and biotechnology , enzyme , financial economics , economics , biology
An isoindolone derivative, Fungi fibrinolytic compound ( R )‐2,5‐bis((2 R ,3 R )‐2‐(( E )‐4,8‐dimethylnona‐3,7‐dien‐1‐yl)‐3,5‐dihydroxy‐2‐methyl‐7‐oxo‐3,4,7,9‐tetrahydropyrano[2,3‐ e ]isoindol‐8(2 H )‐yl)pentanoic acid (FGFC1, Fungi fibrinolytic compound 1 ), was isolated from a rare marine microorganism strain Stachybotrys longispora FG216. The structure of FGFC1 was elucidated by 1 H NMR, 13 C NMR, IR, and MS data; moreover, it was also evaluated for fibrinolytic activity in vitro and in vivo . The results showed that 0.1–0.4 mmol/L of FGFC1 could stimulate generation of plasmin activity (increased by 2.05–11.44 folds) by measuring Glu‐plasminogen and Lys‐plasminogen activation in vitro . The experiment of fluorescein isothiocyanate (FITC)‐fibrinogen degradation indicated that the effect of FGFC1 on fibrinolytic activity was mediated by plasminogen and scuPA. In addition, FGFC1 (10 mg/kg) could dissolve most of pulmonary thrombus of Wistar rat in vivo . It is possible that FGFC1 is a potential thrombolytic agent in the future.