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Self‐assembly of DNA Origami Using Rolling Circle Amplification Based DNA Nanoribbons
Author(s) -
Liu Bing,
Ouyang Xiangyuan,
Chao Jie,
Liu Huajie,
Zhao Yun,
Fan Chunhai
Publication year - 2014
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201300827
Subject(s) - dna origami , rolling circle replication , dna , sticky and blunt ends , base pair , chemistry , dna nanotechnology , helix (gastropod) , nanotechnology , molecular structure of nucleic acids: a structure for deoxyribose nucleic acid , dna replication , materials science , ecology , biochemistry , snail , biology
Abstract During the development of structural DNA nanotechnology, the emerging of scaffolded DNA origami is marvelous. It utilizes DNA double helix inherent specificity of Watson‐Crick base pairing and structural features to create self‐assembling structures at the nanometer scale exhibiting the addressable character. However, the assembly of DNA origami is disorderly and unpredictable. Herein, we present a novel strategy to assemble the DNA origami using rolling circle amplification based DNA nanoribbons as the linkers. Firstly, long single‐stranded DNA from Rolling Circle Amplification is annealed with several staples to form kinds of DNA nanoribbons with overhangs. Subsequently, the rectangle origami is formed with overhanged staple strands at any edge that would hybridize with the DNA nanoribbons. By mixing them up, we illustrate the one‐dimensional even two‐dimensional assembly of DNA origami with good orientation.