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Analysis of Trace‐Level Volatile Compounds in Fresh Turf Crop ( Lolium perenne L.) by Gas Chromatography Quadrupole Time‐of‐Flight Mass Spectrometry
Author(s) -
Hong Saisai,
Kang Wenyu,
Su Yue,
Guo Yinlong
Publication year - 2013
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201300414
Subject(s) - chemistry , mass spectrometry , mass spectrum , electron ionization , gas chromatography , quadrupole time of flight , chromatography , analytical chemistry (journal) , kovats retention index , lolium perenne , mass , ion trap , ion , tandem mass spectrometry , ionization , agronomy , organic chemistry , poaceae , biology
In the traditional research of volatile compounds, some trace‐level compounds could not be identified by gas chromatography‐mass spectrometry. Target and post‐targeted methods were applied in the investigation of trace‐level volatile compounds in fresh turf crop ( Lolium perenne L.) based on gas chromatography in combination with hybrid quadrupole time‐of‐flight mass spectrometry. According to literatures published, a target analysis was performed by using retention index, accurate masses of characteristic ions and second‐stage mass spectra (MS 2 spectra). And a series of experiments showed that low electron impact energy was beneficial to the improvement of the abundances of low abundance molecular ion peak. Enhancing the abundances of low abundance molecular ion peak was beneficial to qualitative analysis. Totally, 60 volatile compounds were identified, the great majority compounds of which were benzeneacetaldehyde (14.8%), 2,5‐dimethyl‐pyrazine (9.6%), and hexanal (9.3%). Identification was complied by mass spectral search, retention index and accurate masses of characteristic ions.