Premium
Fast Determination of Ethylendiamine in Aminophylline Tablets by Small‐sized Capillary Electrophoresis with Amperometric Detection
Author(s) -
Ge Jinyuan,
Tong Fanghong,
Li Ying,
Zhang Yang,
Chu Qingcui,
Ye Jiang
Publication year - 2013
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201300039
Subject(s) - chemistry , capillary electrophoresis , detection limit , amperometry , chromatography , aminophylline , derivatization , electrophoresis , analytical chemistry (journal) , electrode , high performance liquid chromatography , electrochemistry , biochemistry
A novel method for fast determination of ethylendiamine (EDA) in Aminophylline Tablets has been developed by small‐sized capillary electrophoresis with amperometric detection (small‐CE‐AD) coupled with field‐amplified sample injection (FASI). Under the optimum conditions, EDA and four aliphatic diamine homologs (1,3‐diaminopropane, 1,4‐diaminobutane, 1,5‐diaminopentane and 1,6‐diaminohexane) could be well separated within 6 min at a separation voltage of 2.0 kV in an acetate buffer solution of pH 3.8 with low limit of detection (LOD) of 1.3×10 −11 g/mL for EDA ( S / N =3). The proposed method has been successfully applied to direct determination of EDA content in different batches of Aminophylline Tablets. The method does not require off‐line preconcentration and derivatization steps, which should find wide application fields including pharmaceuticals as an alternative to conventional and microchip CE approaches.