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A Near‐infrared Fluorescent Probe for Selective Simultaneous Detection of Fe 2+ and Cl − in Living Cells
Author(s) -
Li Ping,
Xiao Haibin,
Tang Bo
Publication year - 2012
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201200652
Subject(s) - chemistry , cyanine , fluorescence , fluorophore , terpyridine , photoinduced electron transfer , photochemistry , infrared , naked eye , cell permeability , electron transfer , membrane permeability , membrane , analytical chemistry (journal) , biophysics , detection limit , metal , optics , chromatography , organic chemistry , biochemistry , physics , biology
A naked‐eyed chromo‐near infrared fluorescent probe for simultaneous detection of Fe 2+ and Cl − has been developed, based on photoinduced electron transfer mechanism (PET). The fluorophore is cyanine (Cy‐SO 3 − ) and the Fe 2+ receptor is 4′‐(aminomethylphenyl)‐2,2′,6′,2′′‐terpyridine (Tpy). The probe responds linearly and rapidly to [Fe 2+ ] and [Cl − ] variations under physiological conditions and exhibits high sensitivity, good photostability, and excellent cell membrane permeability. The real‐time imaging of cellular Fe 2+ was achieved successfully in living HL‐7702, HepG2, and RAW264.7 cell lines.