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Advantage of Fluorescence Correlation Spectroscopy for the Study of Polyelectrolytes
Author(s) -
Jia Pengxiang,
Gong Yongkuan,
Wang Shengqin,
Zhao Jiang
Publication year - 2012
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201200596
Subject(s) - chemistry , polyelectrolyte , fluorescence correlation spectroscopy , dynamic light scattering , spectroscopy , fluorescence spectroscopy , fluorescence , aqueous solution , chemical physics , dilution , analytical chemistry (journal) , polymer chemistry , polymer , chemical engineering , chromatography , organic chemistry , thermodynamics , molecule , optics , physics , quantum mechanics , nanoparticle , engineering
The advantage of fluorescence correlation spectroscopy to study single chain behavior of polyelectrolytes has been demonstrated by checking the coil‐to‐globule transition of poly 2‐vinylpyridine with the change of pH value in aqueous solution. The ultra‐high sensitivity of FCS allows measurement at extreme dilution where the effect of electrostatic interaction between the chains is greatly suppressed. The results exposed first‐order conformation transition of P2VP as detected by FCS while inter‐chain aggregation occurred in the experiments of dynamic light scattering.