Premium
Highly Sensitive Fluorometric Hg 2+ Biosensor with a Mercury(II)‐Speci?c Oligonucleotide (MSO) Probe and Water‐Soluble Graphene Oxide (WSGO)
Author(s) -
Liu Xingfen,
Miao Likun,
Jiang Xu,
Ma Yanwen,
Fan Quli,
Huang Wei
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201190175
Subject(s) - chemistry , biosensor , graphene , fluorescence , aqueous solution , detection limit , mercury (programming language) , oxide , adsorption , oligonucleotide , photochemistry , inorganic chemistry , nanotechnology , chromatography , organic chemistry , dna , biochemistry , materials science , physics , quantum mechanics , computer science , programming language
A highly sensitive and selective, "turn‐on" and simple Hg 2+ biosensor is reported by using water‐soluble graphene oxide (WSGO) and dye‐labeled mercury(II)‐specific oligonucleotide (MSO) probe. The probe is rich of thymine (T) and can readily form the stem‐loop structure which consists of the T‐Hg 2+ ‐T configuration. In the absence of Hg 2+ , the probe exists as a random coil conformation which can be readily adsorbed on the surface of WSGO by strong noncovalent binding of bases, as a result, the fluorescence of the dye labeled on the terminus of the MSO is strongly quenched by the efficient electron/energy transfer from the dye to WSGO. Upon addition of Hg 2+ , the formation of the T‐Hg 2+ ‐T structure releases the MSO from the surface of WSGO, resulting in a restoration of the fluorescence of dye‐labeled MSO probe. Based on this observation, a highly sensitive and selective Hg 2+ sensor is developed, which can work with "turn‐on" mode in aqueous solutions at room temperature. By using the fluorometric method, the limit of detection for Hg 2+ can reach picomolar range (187 pmol·L −1 ), and it is demonstrated that the biosensor is highly selective and only minimally perturbed by a wide range of non‐specific metal ions.