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Facile Synthesis of Boronic Acid‐Functionalized Magnetic Mesoporous Silica Nanocomposites for Highly Specific Enrichment of Glycopeptides
Author(s) -
Zhang Huaiyuan,
Yao Guoping,
Deng Chunhui,
Lu Haojie,
Yang Pengyuan
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201190166
Subject(s) - chemistry , boronic acid , mesoporous silica , mesoporous material , covalent bond , glycopeptide , horseradish peroxidase , nanocomposite , magnetic nanoparticles , surface modification , combinatorial chemistry , chromatography , organic chemistry , nanoparticle , nanotechnology , materials science , biochemistry , catalysis , enzyme , antibiotics
In the work, aminophenylboronic acid (APB)‐functionalized magnetic mesoporous silica, which holds the attractive features of high magnetic responsivity and large surface area, was developed to enrich glycopeptides. At first, magnetic mesoporous silica nanocomposites were prepared. And then, the nanocomposites were functioned with glycidoxypropyltrimethoxysilane (GLYMO) for boronic acid immobilization. Due to that the boronic acid group on the surface of magnetic mesoporous silica nanocomposites can form tight yet reversible covalent bond with glycopeptides containing cis ‐1,2‐diols groups, the magnetic mesoporous silica nanocomposites were successfully applied to selective enrichment of glycopeptides. APB functionalized magnetic mesoporous silica was also demonstrated to have high selectivity for the glycopeptides in the presence of a 10‐fold excess bovine serum albumin (BSA) over horseradish peroxidase (HRP) in the tryptic digest. We also find that magnetic mesoporous silica has better sensitivity in HRP digest compared with that of commercial aminophenylboronic acid‐functionalized magnetic nanoparticles beads. The limit of detection for glycopeptides from glycoprotein HRP is about 0.01 ng/µL.