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A New and Sensitive Catalytic Resonance Scattering Spectral Assay for the Detection of Laccase Activity Using H 2 O 2 ‐I − ‐TDMAC System
Author(s) -
Liang Aihui,
Wang Pengfei,
Jiang Zhiliang
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201190159
Subject(s) - chemistry , laccase , cationic polymerization , detection limit , catalysis , buffer solution , ammonium chloride , resonance (particle physics) , acetic acid , nuclear chemistry , chloride , inorganic chemistry , chromatography , enzyme , organic chemistry , physics , particle physics
Abstract In pH 3.8 acetic acid‐sodium acetate (HAC‐NaAC) buffer solution, laccase exhibited a strong catalytic effect on the H 2 O 2 oxidation of I − to form I 2 , and I 2 combined with excess I − to form I 3 − that reacted with cationic surfactants of tetradecyl dimethylbenzyl ammonium chloride (TDMAC) to produce the (TDMAC‐I 3 ) n association complex particles, which exhibited a strong resonance scattering (RS) peak at 468 nm. Under the chosen conditions, as the concentration of laccase activity increased, the RS intensity at 468 nm ( I 468 nm ) increased linearly. The increased RS intensity Δ I 468 nm was linear to laccase activity in the range of 0.08–0.96 U/mL, with a regression equation of Δ I 468 nm 88.8 U −1.9, and a detection limit of 0.02 U/mL laccase. This proposed method was applied to detect laccase activity in waste water, with satisfactory results.