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Resonance Scattering Effect of Dopamine Product Particle and Its Application to Polyphenoloxidase Activity Assay
Author(s) -
Jiang Caina,
Ling Shaoming,
Liang Aihui,
Ouyang Xiahui,
Wang Pengfei,
Jiang Zhiliang
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201190120
Subject(s) - chemistry , detection limit , analytical chemistry (journal) , resonance (particle physics) , buffer solution , dopamine , chromatography , nuclear chemistry , physics , particle physics , neuroscience , biology
In pH 7.2 Na 2 HPO 4 ‐NaH 2 PO 4 buffer solution, polyphenoloxidase (PPO) could catalyze the oxidization of dopamine (DA) to form polymer particles in dark‐red color, which exhibited a strong resonance scattering (RS) peak at 780 nm. In the chosen conditions, as the PPO activity increased, the RS intensity at 780 nm increased linearly. The increased RS intensity (Δ I 780 nm ) was linear to the PPO activity in the range of 0.10–6.0 U·mL −1 , with a regression equation of Δ I 780 nm 96.6 C +15.1, a relative coefficient of 0.9987 and a detection limit of 0.06 U·mL −1 PPO. The proposed method was applied to detecting PPO activity in potato sample with satisfactory results.