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Proteomics Analysis of T Lymphocytes Damage Induced by Ionizing Irradiation
Author(s) -
Shi Ying,
Zhang Xuan,
Liu Ning,
Liang Feng,
Song Fengrui,
Liu Zhiqiang,
Liu Shuying
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201190045
Subject(s) - chemistry , proteome , proteomics , apoptosis , ionizing radiation , difference gel electrophoresis , microbiology and biotechnology , jurkat cells , lymphocyte , irradiation , gel electrophoresis , biochemistry , t cell , biology , gene , immune system , immunology , physics , nuclear physics
Abstract Human T lymphocytes were found to be highly radiosensitive and complex cellular responses including apoptosis could be induced upon exposure to X‐ray irradiation. However, the mechanism of apoptosis associated with irradiation was not clear. In this study, a proteomic method was applied to investigation on alteration of proteome of human T‐lymphocyte cells after irradiation. The Jurkat cells were irradiated with 4 Gy X‐ray and the cell lysates were collected at different times after irradiation (6, 12, 18, 24 and 48 h). The whole proteins were separated and quantified by two‐dimensional fluorescence difference gel electrophoresis, and then the differentially expressed proteins were identified by mass spectrometry. 4 proteins exhibited significant irradiation‐induced difference in abundance, including L ‐plastin, bifunctional purine biosynthesis protein, tubulin beta chain, beta‐actin. Differentially expressed proteins were reported to be directly or indirectly involved in the function of human T lymphocyte. Thus, this study might provide clues to identify proteins with biological significance related to irradiation.