Premium
Metabolic Profiling of Human Colorectal Cancer Using High Resolution 1 H Nuclear Magnetic Resonance Spectroscopy
Author(s) -
Chen Wenxue,
Zhou Xiaoyan,
Huang Dan,
Chen Fener,
Du Xiang
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180423
Subject(s) - chemistry , taurine , metabolomics , polyamine , colorectal cancer , nuclear magnetic resonance spectroscopy , malignancy , cancer , metabolome , cancer research , pathology , biochemistry , medicine , biology , amino acid , stereochemistry , chromatography
Colorectal cancer (CRC) is the third commonest malignancy cancer worldwide. Clear understandings of global metabolic profiling of the normal mucosa and cancer tissues are vitally important to aid optimizing the clinical management strategy and understanding CRC biology. We studied metabolic characteristics of 20 CRC and 20 distant normal mucosa tissues extracts from 20 patients using high resolution 1 H NMR spectroscopy in conjunction with multivariate analyses, such as principal component analysis (PCA). Compared with distant normal mucosa tissues, lactate, taurine, ornithine and polyamine were present at significantly higher levels in CRC tissue extracts whereas myo ‐inositol was present at significantly lower level. Two metabolites ratios such as myo ‐inositol/taurine and myo ‐inositol/(ornithine+polyamine) appear to be the most valuable biomarkers for the differentiation CRC from normal mucosa tissues. Our data suggested that HR 1 H NMR spectroscopy combined with multivariate analyses is a potentially useful technology for detecting malignant changes in the normal mucosa tissues, the technique may be further exploited for future CRC biomarker research or identification of targets for therapeutic manipulations.