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Direct Electrochemistry of Hemoglobin Based on Fe 3 O 4 @SiO 2 Nanoparticles Modified Electrode
Author(s) -
Cui Rongjing,
Yin Fan,
Zhou Lijuan,
Pan Hongcheng
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180420
Subject(s) - chemistry , high resolution transmission electron microscopy , biosensor , analytical chemistry (journal) , cyclic voltammetry , amperometry , indium tin oxide , nanoparticle , fourier transform infrared spectroscopy , electrode , detection limit , selectivity , electrochemistry , transmission electron microscopy , nuclear chemistry , nanotechnology , chemical engineering , materials science , chromatography , organic chemistry , biochemistry , engineering , catalysis
A biosensor based on hemoglobin‐Fe 3 O 4 @SiO 2 nanoparticle bioconjunctions modified indium‐tin‐oxide (Hb/Fe 3 O 4 @SiO 2 /ITO) electrode was fabricated to determine the concentration of H 2 O 2 . UV‐vis absorption spectra, fourier transform infrared (FT‐IR) spectroscopy, cyclic voltammetry (CV) and high‐resolution transmission electron microscopy (HRTEM) were used to characterize the bioconjunction of Fe 3 O 4 @SiO 2 with Hb. Experimental results demonstrate that the immobilized Hb on the Fe 3 O 4 @SiO 2 matrix retained its native structure well. In addition, Fe 3 O 4 @SiO 2 nanoparticles (NPs) are very effective in facilitating electron transfer of the immobilized enzyme, which can be attributed to the unique nanostructure and larger surface area of the Fe 3 O 4 @SiO 2 NPs. The biosensor displayed good performance for the detection of H 2 O 2 with a wide linear range from 2.03×10 −6 to 4.05×10 −3 mol/L and a detection limit of 0.32 µmol/L. The resulting biosensor exhibited fast amperometric response, good stability, reproducibility, and selectivity to H 2 O 2 .