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Effect of Temperature on Self‐Interaction of Human‐Like Collagen
Author(s) -
Fan Daidi,
Xing Jianyu,
Xue Wenjiao,
Zhu Chenhui,
Ma Xiaoxuan,
Ma Rong
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180318
Subject(s) - chemistry , hydrogen bond , hydrophobic effect , monomer , atmospheric temperature range , sodium , molecule , polyacrylamide gel electrophoresis , chromatography , polymer chemistry , chemical engineering , organic chemistry , polymer , enzyme , thermodynamics , physics , engineering
Effects of temperature on self‐interaction of human‐like collagen (HLC) were investigated by hydrophobic interaction chromatography, calorimetric measurement, and sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) analysis. Results show that three types of interaction roles may exist between HLC molecules at 3–50°C, which were divided into three narrower temperature ranges. In temperature range from 3–22°C, hydrogen bonding plays a key role in the formation of a gelatinous aggregate. In the range of 22–38°C, hydrophobic bonds accompanied by hydrogen bonds are involved in the formation compact aggregates. When temperature is above 38°C the hydrophobic effect formed in the HLC monomer results in the loss of its ability to self‐interact.