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Determination of Amantadine Residue in Honey by Solid‐phase Extraction and High‐performance Liquid Chromatography with Pre‐column Derivatization and Fluorometric Detection
Author(s) -
Zhang Jinzhen,
Zhao Jing,
Zhou Jinhui,
Xue Xiaofeng,
Li Yi,
Wu Liming,
Chen Fang
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180313
Subject(s) - chemistry , chromatography , derivatization , detection limit , trifluoroacetic acid , high performance liquid chromatography , solid phase extraction , residue (chemistry) , acetonitrile , extraction (chemistry) , amantadine , organic chemistry , neuroscience , biology
Amantadine (AMA) is an anti‐viral drug used in apiculture to protect honeybee against the sacbrood virus ( Morator aetatulae ). This study described a reliable high‐performance liquid chromatographic (HPLC) method for analyzing AMA in honey using a solid‐phase extraction (SPE) cartridge (Plexa PCX) for purification, 4‐fluoro‐7‐nitro‐2,1,3‐benzoxadiazole (NBD‐F) as a pre‐column derivatization agent, and fluorometric detection ( λ ex =470 nm, λ em =530 nm). The chromatographic separation was performed on an XDB C18 column (150×4.6 mm i.d.) using 0.1% trifluoroacetic acid/acetonitrile (35:65, V / V ) as the mobile phase at a flow rate of 1.0 mL·min −1 with a run time of 20 min. Under these optimal conditions, a linear relationship was observed in the range of 0.025–1.0 µg· mL −1 with a good correlation coefficient (0.998) and low limit of detection (0.0080 µg·g −1 ), the recoveries were all above 90%, and the intra‐day and inter‐day precision (RSD) ranged from 3.4%–5.1%.