Extraction and Determination of Quercetin and Myricetin from Chamaecyparis obtusa by Ionic Liquids‐based Monolithic Cartridge

A short ionic liquids (ILs)‐based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission‐scanning electron microscopy (FE‐SEM), a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa ( C. obtusa ) by using ILs‐based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C 18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile‐water (25/75, V/V ) as the mobile phase, and a flow rate of 0.7 mL·min −1 . A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5–100.0 µg·mL −1 . The recoveries ranged from 101.6% to 104.6% and the inter‐ and intra‐day relative standard deviations (RSD) were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants.