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Proteomics‐based Characterization of Protein Complexes from Human Pancreatic Cancer Cell Line
Author(s) -
Wang Xinli,
Chen Guoqiang,
Zhao Zhiyun,
Wang Xiaodong,
Li Zhili
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180278
Subject(s) - chemistry , proteomics , lysis , differential centrifugation , polyacrylamide gel electrophoresis , gel electrophoresis , cell culture , centrifugation , pancreatic cancer , biochemistry , microbiology and biotechnology , cancer , enzyme , medicine , biology , gene , genetics
To develop an economical and feasible approach to probe protein complexes, differential centrifugation and three‐dimensional polyacrylamide gel electrophoresis (PAGE) were performed to separate protein complexes from the cell lysate of human pancreatic cancer cell line, SW1990, followed by mass spectrometric identification. Four macromolecular protein complexes were separated and identified unambiguously.