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Antibacterial Activities and Nuclease Properties of Two New Ternary Copper(II) Complexes with 2‐(4′‐Thiazolyl)‐benzimidazole and 2,2′‐Bipyridine/1,10‐phenanthroline
Author(s) -
Ren Xiangxiang,
Chen Jiayang,
Le Xueyi
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180257
Subject(s) - chemistry , molar conductivity , agarose gel electrophoresis , phenanthroline , cyclic voltammetry , copper , crystallography , phenazine , bipyridine , benzimidazole , stereochemistry , medicinal chemistry , dna , crystal structure , metal , organic chemistry , electrochemistry , biochemistry , electrode
Two new complexes: [Cu(TBZ)(bipy)Cl]Cl·H 2 O ( 1 ) and [Cu(TBZ)(phen)Cl]Cl·H 2 O ( 2 ) [TBZ=2‐(4′‐thiazolyl)‐ benzimidazole, phen=1,10‐phenanthroline and bipy=2,2′‐bipyridine] have been synthesized and characterized by elemental analysis, molar conductivity, IR, and UV‐vis methods. Complex 2 , structurally characterized by single‐crystal X‐ray crystallography, crystallizes in the monoclinic space group P 2 1 / c in a unit cell of a =0.85257(12) nm, b =2.5358(4) nm, c =1.15151(13) nm, β =118.721(8)°, V =2.183.2(5) nm 3 , Z =4, D c =1.624 g·cm −3 , µ =1.367 mm −1 . The complexes, free ligands and chloride copper(II) salt were each tested for their ability to inhibit the growth of two gram‐positive ( B. subtilis and S. aureus ) and two gram‐negative ( Salmonella and E. coli ) bacteria. The complexes showed good antibacterial activities against the microorganisms. The interaction between the complexes and calf thymus DNA in aqueous solution was investigated adopting electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and cyclic voltammetry. Results suggest that the two complexes can bind to DNA by intercalative mode. In addition, the result of agarose gel electrophoresis suggested that the complexes can cleave the plasmid DNA at physiological pH and room temperature. Mechanistic studies with different inhibiting reagents reveal that hydroxyl radicals, and a singlet oxygen‐like copper‐oxo species are all involved in the DNA scission process mediated by the complexes.