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Study on the Interaction of Vitamin B 12 with DNA by Spectroscopy and Electrochemical Methods
Author(s) -
Hajian Reza,
Zafari Mitra
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180254
Subject(s) - chemistry , binding constant , cyclic voltammetry , dna , methylene , methylene blue , electrochemistry , voltammetry , molecule , analytical chemistry (journal) , glassy carbon , electrode , crystallography , stereochemistry , binding site , organic chemistry , biochemistry , photocatalysis , catalysis
The Interaction between vitamin B 12 (VB 12 ) and fish sperm DNA was investigated in physiological buffer (pH 7.4) using the methylene blue (MB) dye as a spectral probe by spetcrophotometery, viscosity measurements and cyclic voltammetry. The apparent binding constant of vitamin B 12 with DNA was found to be 3.2×10 5 mol −1 ·L. The voltammetric behavior of vitamin B 12 has been investigated at glassy carbon electrode using cyclic voltammetry. Thermodynamic parameters including Δ H 0 , Δ S 0 and Δ G 0 for the interaction between VB 12 and DNA have determined as −2.3×10 4 , 27.54 and −3.1×10 4 J·mol −1 ·K −1 respectively. One indication of DNA binding mode with VB 12 was the change in viscosity when a small molecule associates with DNA. The diffusion coefficients of VB 12 in the absence ( D 0 ) f and presence of DNA ( D 0 ) b was calculated as 5.04×10 −6 and 1.13×10 −6 cm 2 ·s −1 respectively. The results indicated that vitamin B 12 can bind to DNA and the major binding mode was intercalative binding.