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Lanthanum Chloride Promoted Proliferation with Enhanced S‐phase Entry and Inhibited Potassium Currents of NIH 3T3 Cells
Author(s) -
Zhang Liping,
Li Zhuoyu,
Yang Pin
Publication year - 2011
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201180247
Subject(s) - chemistry , intracellular , cell growth , 3t3 cells , potassium , lanthanum , microbiology and biotechnology , cell , biophysics , cell cycle , apoptosis , fibroblast , biochemistry , inorganic chemistry , biology , in vitro , transfection , organic chemistry , gene
The effects of La 3+ on proliferation, cell cycles, apoptosis and ion channels were investigated in mouse embryo fibroblast NIH 3T3 cells and its possible mechanisms were explored. Our data showed that La 3+ promoted cell proliferation with increased S‐phase entry and inhibited the outward potassium currents in a concentration‐dependent manner in NIH 3T3 cells. La 3+ and Ca 2+ had synergistic effect on cell proliferation and cell cycles. It showed that Ca 2+ was needed for La 3+ ‐promoted cell cycle progression. Using the whole‐cell voltage‐clamp technique, we found that La 3+ blocked the outward potassium current in a concentration‐dependent manner in NIH 3T3 cells. Lanthanum ions can increase intracellular Ca 2+ concentration through inhibition of potassium currents, which induce a series of physiological changes and improve proliferation of cells. This may be one of the molecular mechanisms of lanthanum ions induced cell proliferation. The present work provides a new perspective for understanding the biological and toxicological effects of lanthanum.