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Rapid Determination of Costunolide and Dehydrocostuslactone in Human Plasma Sample and Chinese Patent Medicine Xiang Sha Yang Wei Capsule Using HPLC‐DAD Coupled with Second‐order Calibration
Author(s) -
Liu Yajuan,
Wu Hailong,
Zhu Shaohua,
Kang Chao,
Xu Hui,
Su Zhiyi,
Gu Huiwen,
Yu Ruqin
Publication year - 2012
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201100677
Subject(s) - chemistry , capsule , high performance liquid chromatography , chromatography , analyte , calibration , sample (material) , human plasma , analytical chemistry (journal) , statistics , botany , mathematics , biology
A novel methodology that combines high performance liquid chromatography with photodiode‐array detector (HPLC‐DAD) coupled with second‐order calibration method based on alternating trilinear decomposition (ATLD) algorithm was used in determination of the effective constituents such as costunolide and dehydrocostuslactone, in plasma sample and Chinese patent medicine Xiang Sha Yang Wei (XSYW) capsule. Complicated systems such as plasma and Chinese patent medicine which have intricate components are tedious to isolate and purify. The problem that chromatographic peaks are heavily overlapped among the analytes and interferents from the background matrices can be resolved, and the satisfactory quantification results have been gained with the help of the ATLD algorithm which utilized "mathematical separation" instead of partial "physical or chemical separation". Meanwhile, HPLC‐MS/MS method was used to validate the accuracy of the proposed determination method.