Premium
A Highly Sensitive Electrochemical Impedance Spectroscopy Immunosensor for Determination of 1‐Pyrenebutyric Acid Based on the Bifunctionality of Nafion/Gold Nanoparticles Composite Electrode
Author(s) -
Yang Ping,
Zheng Qiaoli,
Xu He,
Liu Jianshe,
Jin Litong
Publication year - 2012
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201100485
Subject(s) - nafion , dielectric spectroscopy , detection limit , electrode , chemistry , colloidal gold , nanoparticle , electrochemistry , analytical chemistry (journal) , nuclear chemistry , materials science , chromatography , nanotechnology
A simple, highly sensitive and label‐free electrochemical impedance spectroscopy (EIS) immunosensor was developed using Nafion and gold nanoparticles (nano‐Au/Nafion) composites for the determination of 1‐pyrenebutyric acid (PBA). Under the optimal conditions, the amount of immobilized antibody was significantly improved on the nano‐Au/Nafion electrode due to the synergistic effect and biocompatibility of Nafion film and gold nanoparticles composites. The results showed that the sensitivity and stability of nano‐Au/Nafion composite electrode for PBA detection were much better than those of nano‐Au modified glassy carbon electrode (nano‐Au/GCE). The plot of increased electron transfer resistances ( R ets ) against the logarithm of PBA concentration is linear over the range from 0.1 to 150 ng·mL −1 with the detection limit of 0.03 ng·mL −1 . The selectivity and accuracy of the proposed EIS immunosensor were evaluated with satisfactory results.