Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross‐linking | Zendy

Liu Yuge | Zendy; Li Weiming | Zendy; Wei Changbin | Zendy; Lü Lingling | Zendy

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Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross‐linking

Author(s) -

Liu Yuge,

Li Weiming,

Wei Changbin,

Lü Lingling

Publication year - 2012

Publication title -

chinese journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.28

H-Index - 41

eISSN - 1614-7065

pISSN - 1001-604X

DOI - 10.1002/cjoc.201100477

Subject(s) - chemistry , xanthine , xanthine oxidase , glutaraldehyde , hypoxanthine , chitosan , electrode , biosensor , detection limit , nuclear chemistry , electrochemistry , selectivity , immobilized enzyme , chromatography , organic chemistry , enzyme , biochemistry , catalysis

Here in this paper, xanthine oxidase (XOD) was immobilized onto the chitosan (CHT) modified electrode by a simple way of cross‐linking with glutaraldehyde (GTD) and 3‐aminopropyltriethoxysilane (KH). The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 μmol/L with a low detection limit of 0.0215 µmol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxanthine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.

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