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Study on the Metabolic Characteristics of Aconite Alkaloids in the Extract of Radix aconiti under Intestinal Bacteria of Rat by UPLC/MS n Technique
Author(s) -
Xin Yang,
Pi Zifeng,
Song Fengrui,
Liu Zhiqiang,
Liu Shuying
Publication year - 2012
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201100228
Subject(s) - aconitine , chemistry , radix (gastropod) , chromatography , aconitum , alkaloid , high performance liquid chromatography , traditional medicine , stereochemistry , medicine , botany , biology
The extract of Radix aconiti was incubated with rat intestinal bacteria in vitro . Further, aconitine and hypaconitine standard were incubated at the same condition as the extract of Radix aconiti , respectively. Ultra Performance Liquid Chromatography/Multi‐Mass Spectrometry (UPLC/MS n ) was used for detecting and identifying all the aconite alkaloids. Results showed that there were four metabolites which were identified as 8‐butyryl‐14‐benzoylmesa‐conine ( m/z 660), 8‐propionyl‐14‐benzoylaconine ( m/z 660), 8‐butyryl‐14‐benzoylaconine ( m/z 674) and 8‐valeryl‐14‐benzoylmesaconine ( m/z 674) in the metabolized sample of the extract of Radix aconiti . The relative area ratio of them presented increasing trend during 6 d. On the basis of all results, we could concluded that substitution at N atom mainly influenced the metabolizing rate of diester‐diterpenoid alkaloids (DDAs), C‐8 substitute was active metabolized site, intestinal bacterial metabolites of the aconite alkaloids in the extract of Radix aconiti were mainly the substitute with propionyl group, butyl group or valeryl group at C‐8. This paper illustrated holistic metabolizing profile of the extract of Radix aconiti in vitro and possible metabolizing reaction type of main DDAs, which could provide reference for finding out potential bioactive components in the extract and the prescription of Chinese Medicine.