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Development of High Performance Liquid Chromatography Method for Costunolide and Dehydrocostuslactone in Mice Plasma and Tissues: Application to Pharmacokinetic Study
Author(s) -
Hu Fangdi,
Feng Shilan,
Wu Yuqiong,
Bi Yingyan,
Cui Fang,
Li Yongjie,
Wang Chunming
Publication year - 2010
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201090379
Subject(s) - chemistry , chromatography , high performance liquid chromatography , extraction (chemistry) , pharmacokinetics , acetonitrile , pharmacology , medicine
A simple and sensitive high performance liquid chromatographic (HPLC) method for simultaneous determination of costunolide and dehydrocostuslactone in mice plasma and tissues was developed and validated. Costunolide and dehydrocostuslactone were extracted into acetonitrile and separated using an isocratic mobile phase, on a Hypersil ODS C18 column. The effluent was monitored by UV detector at 210 nm and at a flow rate of 1.0 mL· min −1 and 25°C. The linearity ranges of proposed method were 0.223–8.920 µg·mL −1 for costunolide and 0.227–9.080 µg·mL −1 for dehydrocostuslactone. The intra‐day and inter‐day RSD of the assay method for the two components were less than 5%, and mean recovery was within the 86.5% to 101.8% range. The method was found to be precise, accurate, and specific during the study. The method was successfully applied for pharmacokinetic study of costunolide and dehydrocostuslactone after application of ethanol extraction of Muxiang (EEM) in mice.