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Design and Synthesis of Biotinylated Troglitazone as an Active Affinity Probe
Author(s) -
Li Xin,
Li Xiaoqi,
Tang Hong,
Nan Fajun
Publication year - 2010
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201090370
Subject(s) - biotinylation , troglitazone , chemistry , biotin , hek 293 cells , combinatorial chemistry , membrane , affinity chromatography , biochemistry , receptor , enzyme , peroxisome
A biotin‐tagged analogue of troglitazone was designed, synthesized and applied to affinity chromatography to pulldown EGFR from 293T cell lysates overexpressing EGFR, a membrane protein assumed to be troglitazone's direct binding target by previous work. The results indicate the feasibility of the biotinylated probe as a vigorous tool to clarify the molecular mechanisms for troglitazone's antitumor activities.