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Point Mutation Genotyping Based on Melting‐Point Difference without Fluorescent Probes
Author(s) -
Meng Xuan,
Huang Jianhua,
Meng Xiangxian,
Wu Yanqiong,
Mo Qiuhua
Publication year - 2010
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201090348
Subject(s) - genotyping , point mutation , fluorescence , chemistry , melting point , genotype , melting curve analysis , significant difference , mutation , microbiology and biotechnology , polymerase chain reaction , biochemistry , biology , optics , physics , gene , organic chemistry , statistics , mathematics
Fluorescent method is a powerful tool for genotyping point mutations. Current existing methods usually need cost labeled fluorescent probes, which causes great limitation to their applications in clinic. Here, a fidelity method for genotyping point mutations has been developed based on melting‐point difference without labeled fluorescent probes. The method employs ligase to ligate two specific probes to produce a high melting temperature. The homozygotes and heterozygotes are scored accurately by obvious melting‐point difference among genotypes. This method would provide an accurate and economical‐cost tool for point mutation genotyping.