Premium
Recognizing Hybrid/Mixed G‐quadruplex in Human Telomeres by Using a Cyanine Dye Supramolecule with Confocal Laser Scanning Microscopy
Author(s) -
Yang Qianfan,
Xiang Junfeng,
Yang Shu,
Zhou Qiuju,
Li Qian,
Guan Aijiao,
Zhang Xiufeng,
Zhang Hong,
Tang Yalin,
Xu Guangzhi
Publication year - 2010
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201090196
Subject(s) - chemistry , cyanine , g quadruplex , telomere , dna , supramolecular chemistry , confocal , confocal microscopy , in vitro , biophysics , duplex (building) , crystallography , biochemistry , fluorescence , microbiology and biotechnology , crystal structure , physics , geometry , mathematics , quantum mechanics , biology
Abstract Single‐stranded telomeric DNA tends to form a four‐base‐paired planar structure termed G‐quadruplex. Although kinds of G‐quadruplex structures in vitro have been documented in the presence of potassium or sodium, recognition of these DNA motifs (both in vitro and in vivo ) is still an important issue in understanding the biological function of the G‐quadruplex structures in telomeres as well as developing anticancer agents. Herein we address this important question through the distinctive properties of a supramolecular system of cyanine dye 3,3′‐di(3‐sulfopropyl)‐4,5,4′,5′‐dibenzo‐9‐methyl‐thiacarbocyanine triethylammonium salt (MTC) upon binding to different DNA motifs. Interaction of MTC with hybrid/mixed G‐quadruplex results in a set of unique spectrophotometric signatures which are completely different from those arising from binding to other DNA motifs. Furthermore, such feature could be extended to map the locations of DNAs on interface. Linear duplex and mixed G‐quadruplex in human telomeres assembled on Au film and stained by MTC were directly recognized by confocal laser scanning microscopy (CLSM). All results suggested that MTC supramolecular system may be a good probe of specific G‐quadruplex structure.