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Coupling Proteins to Amine‐terminated Magnetic Particles Activated with 1,4‐Phenylene Diisothiocyanate
Author(s) -
Tang Yitong,
Geng Tingting,
Xin Xiaofang,
Chen Chao,
Cui Yali
Publication year - 2010
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.201090124
Subject(s) - chemistry , magnetic nanoparticles , amine gas treating , streptavidin , fourier transform infrared spectroscopy , micrography , chemical engineering , scanning electron microscope , nanoparticle , organic chemistry , biotin , materials science , biochemistry , composite material , engineering
The homobifunctional crosslinker 1,4‐phenylene diisothiocyanate (PDC) was coupled to amine‐terminated magnetic particles, and human IgG, streptavidin, protein G and protein A were immobilized on the activated magnetic particles. The coupling of PDC to the amine‐terminated magnetic particles was completed in 10 min, and 1 mg of activated magnetic particles was able to immobilize 95 (g of protein G, 120 µg of protein A, 160 µg of streptavidin and 280 µg of IgG. Ultraviolet‐visible spectroscopy, FTIR spectroscopy and electron micrography were used to characterize the functional particles. The results indicated that PDC was successfully coupled to the surface of the amine‐terminated magnetic particles, and the proteins were effectively immobilized on their surface. The activity of protein G immobilized on the activated magnetic particles was confirmed by its ability to purify IgG from plasma.