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Determination of Three Tanshinones from Radix Salvia Miltiorrhiza by Molecularly Imprinted Solid‐phase Extraction
Author(s) -
Yan Hongyuan,
Tian Minglei,
Row Kyung Ho
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990371
Subject(s) - salvia miltiorrhiza , chemistry , radix (gastropod) , chromatography , extraction (chemistry) , salvia , high performance liquid chromatography , molecularly imprinted polymer , trifluoroacetic acid , detection limit , solid phase extraction , methanol , traditional medicine , selectivity , organic chemistry , medicine , botany , alternative medicine , pathology , traditional chinese medicine , catalysis , biology
A selective molecularly imprinted solid‐phase extraction procedure was developed for the selective separation of tanshinone I, tanshinone IIA, and cryptotanshinone from Radix Salvia Miltiorrhiza samples. Tanshinone IIA imprinted polymers (MIP) synthesized in ethanol‐dodecanol system show high affinity to tanshinone IIA and its structure analogs in aqueous environment and the affinity can be controlled by adjusting the intensity of the eluents. By using 60% water‐40% methanol (volume ratio) and 99.5% methanol‐0.5% trifluoroacetic acid (volume ratio) as washing and eluting solvents, most interferences originating from the salvia matrix were eliminated. The extracts were sufficiently clean enough to be directly injected into HPLC for further chromatographic analysis. Good linearity was obtained from 0.4 to 500.0 mg·L −1 ( r 2 =0.999) with the relative standard deviations less than 4.2%. The mean recoveries of tanshinone IIA in Radix Salvia Miltiorrhiza were more than 85.6% at three different concentrations and the limits of detection were 0.06–0.09 mg·L −1 . This method is a viable alternative tool to the existing HPLC methods for analyzing the content of the three tanshinones in Radix Salvia Miltiorrhiza samples.