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DNA Separation by Microchip Electrophoresis Using Copolymers of Poly(vinylpyrrolidone) and Hydroxyethylcellulose
Author(s) -
Li Gang,
Ni Xiangfang,
Dong Shuqing,
Wang Qingjiang,
He Pinggang,
Fang Yuzhi
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990370
Subject(s) - copolymer , chemistry , haeiii , molar ratio , polymer , chromatography , electrophoresis , dna , capillary electrophoresis , polymer chemistry , organic chemistry , polymerase chain reaction , biochemistry , restriction fragment length polymorphism , catalysis , gene
Copolymers of poly(vinylpyrrolidone) (PVP) and hydroxyethylcellulose (HEC) were synthesized, with PVP to HEC molar ratios of 3:1, 2:1 and 1:1. The copolymers were tested as separation media in DNA fragment separation analysis by microchip electrophoresis (MCE). Separation efficiency over 3.8×10 5 for 118 bp has been reached by using the bare channels without the additional polymer coating step. Under optimized separation conditions for longer read length DNA sequencing, the separation ability of the copolymers decreased with decreasing (PVP‐ co ‐HEC) molar ratio from 3:1 to 2:1 and 1:1. In comparison with (PVP‐ co ‐HEC) 1:1, the copolymer with (PVP‐ co ‐HEC) 3:1 ratio showed high separation efficiency. By using a 20 g·L −1 copolymer with (PVP‐ co ‐HEC) 3:1 ratio, ΦΧ174‐HaeIII digest DNA marker was successfully separated within 3 min.